Anti-MTX2 Polyclonal Antibody for WB, IF/ICC, ELISA - O75431

Core Product Specifications and Parameters

Biological Background: Metaxin-2 Function and Localization

• Metaxin-2 is a mitochondrial outer membrane protein that facilitates the import of precursor proteins into the mitochondrion, playing a critical role in mitochondrial biogenesis.
• It is also designated as Mitochondrial outer membrane import complex protein 2, reflecting its integral participation in the protein translocation machinery.
• The MTX2 gene encodes a 29763 Da protein (UniProt calculated molecular weight), which may be subject to alternative splicing and acetylation, as indicated by UniProt keywords.
• Subcellular localization studies confirm metaxin-2 resides at the mitochondrial outer membrane and within the mitochondrion, consistent with its transport function.
• Keywords associate MTX2 with protein transport and osteoporosis, hinting at potential roles in mitochondrial dysfunction and bone metabolism.
• The protein’s cross-reactivity across human, mouse, and rat underscores its evolutionary conservation and importance in mitochondrial protein import.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1–263 of human MTX2, covering the N-terminal region; the polyclonal nature of the antibody suggests recognition of multiple epitopes within this domain.
• For Western blot, a band around 30 kDa is expected; however, actual migration may vary due to post-translational modifications or sample preparation. Include appropriate controls and validate the observed band in your experimental system.
• In immunofluorescence or immunocytochemistry, anticipate a mitochondrial staining pattern. Co-staining with a mitochondrial marker (e.g., MitoTracker) is recommended to confirm localization.
• For ELISA, consider testing a range of antigen concentrations and antibody dilutions to optimize signal-to-noise ratio for your specific assay format.
• The antibody is reported to react with human, mouse, and rat samples; verify cross-reactivity in your target tissue or cell line of interest before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio that includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—all from a single partner. This anti-MTX2 polyclonal antibody supports mitochondrial protein import research and related diagnostic development. Let CamelBio’s one-stop supply chain accelerate your project from concept to clinic with reliable, high-quality reagents.