Anti-MTUS2 Polyclonal Antibody (TIP150) for WB & ELISA - Q5JR59

Core Product Specifications and Parameters

Biological Background: MTUS2 Function and Localization

• Protein identity: MTUS2 (microtubule-associated tumor suppressor candidate 2) is also known as TIP150 (tracking protein of 150 kDa) and cardiac zipper protein (CAZIP). It is encoded by the MTUS2 gene in humans.
• Core function: MTUS2 binds to microtubules and, together with MAPRE1, may target the microtubule depolymerase KIF2C to the plus-end of microtubules, thereby regulating microtubule dynamics at their growing distal tips.
• Subcellular localization: MTUS2 is localized to the cytoplasm and associates with the cytoskeleton, consistent with its role in microtubule plus-end tracking.
• Tissue specificity: The protein has been detected in embryonic stem cells undergoing differentiation into cardiomyocytes, suggesting a role in cardiac development.
• Keywords and features: The protein contains coiled-coil domains, undergoes alternative splicing, and is classified as a reference proteome member. Its molecular weight is approximately 150 kDa.
• Tumor suppression: As a microtubule-associated tumor suppressor candidate, MTUS2 may play a role in cell cycle regulation and tumor suppression, though the exact mechanisms remain to be fully elucidated.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant protein corresponding to amino acids 49–348 of human MTUS2, a region that may confer specificity for MTUS2 over other microtubule-associated proteins.
• Western blotting: Anticipate a predominant band near 150 kDa in lysates from human, mouse, or rat cells, reflecting the full-length protein. Consider using protease inhibitors during sample preparation, and validate band identity with appropriate controls.
• ELISA application: This polyclonal antibody can be employed in both direct and indirect ELISA formats. Titration and optimization are recommended to determine the optimal working concentration for your specific assay.
• Cross-reactivity: The antibody shows cross-reactivity with mouse and rat MTUS2, making it suitable for comparative studies across species. However, always confirm cross-reactivity in your own experimental systems.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti-MTUS2 antibody. For your microtubule and tumor suppressor research programs, we also offer optimized monoclonal antibodies, recombinant proteins, and bulk ancillary reagents. Our sourcing expertise extends to rare targets and custom production, providing a reliable bridge from concept to clinic.