Anti-MTTP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P55157

Core Product Specifications and Parameters

Biological Background: MTTP Function and Localization

• Protein identity: The MTTP gene encodes the large subunit of microsomal triglyceride transfer protein (also known as MTP or ABL), a key player in lipid transport and lipoprotein assembly.
• Core biochemical activity: MTTP catalyzes the transfer of triglyceride, cholesteryl ester, and phospholipid between membrane surfaces, a process essential for the formation of apolipoprotein B-containing lipoproteins. Related references: PMID:15897609, PMID:16478722, PMID:22236406, PMID:23475612, PMID:26224785
• Subcellular localization: MTTP resides in the endoplasmic reticulum and Golgi apparatus, where it loads lipids onto nascent apolipoprotein B particles.
• Tissue distribution: Highest expression in liver and small intestine, with additional presence in ovary, testis, and kidney, reflecting its role in dietary fat absorption and hepatic lipoprotein secretion.
• Structural features: The protein contains a signal peptide, lipid-binding domains, and disulfide bonds; alternative splicing produces multiple isoforms. The 3D structure is known and aids drug targeting.
• Clinical relevance: Mutations in MTTP cause abetalipoproteinemia, a rare disorder marked by the inability to produce chylomicrons and VLDL, leading to severe fat malabsorption.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 550–650 of human MTTP; this region is likely accessible and specific, supporting detection in Western blot (WB), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA.
• For WB, the predicted molecular weight is ~99 kDa; validate the band in your lysate and consider using a reducing loading buffer to address potential disulfide bonds.
• In IF/ICC, permeabilization is essential for detecting MTTP in the ER and Golgi. Perform fixation/permeabilization optimization to preserve these organelles.
• The antibody cross-reacts with mouse and rat MTTP, enabling multi-species studies; always verify specificity in the relevant tissue (e.g., liver, small intestine) with appropriate controls.
• For ELISA applications, confirm coating and blocking conditions empirically; the synthetic peptide immunogen may serve as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-MTTP antibody supports research in lipid metabolism and lipoprotein disorders, and we can source rare-target raw materials or custom-developed antibodies to meet your specific assay requirements. Whether you need ready-to-use reagents or tailored supply solutions, partner with CamelBio to accelerate your diagnostic development.