Anti-MTSS1 Rabbit Polyclonal Antibody for WB and ELISA - O43312

Core Product Specifications and Parameters

Biological Background: MTSS1 Function and Localization

• Protein MTSS 1 (Metastasis suppressor protein 1), also referred to as MIM (Missing in Metastasis), MIMA, MIMB, is a cytoskeleton-associated protein encoded by the MTSS1 gene (GeneID: 9788) in humans. It belongs to the MTSS family and contains an actin-binding domain along with a coiled-coil region.
• MTSS1 is implicated in the regulation of cancer progression and tumor metastasis, likely through its interaction with the actin cytoskeleton, thereby influencing cell motility and morphology.
• The protein is expressed in a wide range of human tissues, with notable presence in spleen, thymus, prostate, testis, uterus, colon, and peripheral blood, as documented by UniProt.
• Subcellularly, MTSS1 localizes predominantly to the cytoplasm and associates with the actin cytoskeleton, consistent with its role in actin dynamics.
• Key post-translational modifications include phosphorylation (evidenced by its classification as a phosphoprotein) and alternative splicing, which may generate isoforms with distinct functional properties.
• Functional classifications from UniProt highlight its involvement as an actin-binding protein, tumor suppressor, and its identification in proteomics studies, underscoring its significance in cell biology and disease.

Experimental Guidance and Technical Tips

• The immunogen used to generate this polyclonal antibody is a recombinant protein corresponding to amino acids 630-730 of human MTSS1 (NP_055566.3). Researchers should confirm specificity by peptide competition assays or by testing in MTSS1-knockdown/-knockout cell models.
• This antibody is suitable for Western blot (WB) and ELISA applications. For WB, a typical starting dilution range may be explored, and optimization is recommended based on target abundance and sample type. Consider using a 5% non-fat milk or BSA blocking buffer.
• As a rabbit polyclonal antibody, it may recognize multiple MTSS1 isoforms resulting from alternative splicing. Run appropriate positive and negative controls, and verify band sizes against predicted molecular weights (~82 kDa).
• For ELISA, plate coating conditions and detection system should be optimized empirically; the antibody can be used as both capture and detection reagent if paired with a suitable conjugate, though independent validation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers, laboratories, and research institutes, supplying validated raw materials for immunoassay development. This MTSS1 polyclonal antibody supports research in cancer metastasis and cytoskeletal biology, and can be integrated into assay workflows. Beyond individual antibodies, CamelBio provides matched antibody pairs, bulk reagents, and customized sourcing solutions for rare or challenging targets, streamlining your path from concept to clinic.