Anti-MTRR Rabbit Polyclonal Antibody for WB, ELISA - Q9UBK8

Core Product Specifications and Parameters

Biological Background: MTRR Function and Localization

• MTRR is a key enzyme in methionine and folate homeostasis, catalyzing the reductive methylation of methionine synthase (MTR)-bound cob(II)alamin to regenerate active MTR.
  Related references:
  PMID:17892308
• It also acts as a molecular chaperone for MTR, stabilizing the apoenzyme and incorporating methylcob(III)alamin to form the functional holoenzyme.
  Related references:
  PMID:16769880
• MTRR possesses aquacob(III)alamin reductase activity, reducing aquacob(III)alamin to cob(II)alamin and thereby promoting MTR holoenzyme assembly.
  Related references:
  PMID:16769880
• In the cytoplasm, MTRR functions within a multiprotein complex that includes MMACHC, MMADHC, and MTR, ensuring efficient cobalamin processing.
  Related references:
  PMID:27771510
• MTRR is widely expressed across tissues, with particularly high abundance in skeletal muscle.
• The protein contains binding sites for cofactors FAD, FMN, and NADP, and its activity is modulated by phosphorylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 50–230 of human MTRR, an internal region expected to detect full-length protein in relevant applications.
• For Western blot, consider using reducing conditions and a positive control lysate from a tissue with high MTRR expression (e.g., skeletal muscle); the predicted molecular weight is approximately 78 kDa.
• In ELISA, the antibody may be used with recombinant MTRR protein or peptide; titrate the antibody to optimize signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, helping diagnostic manufacturers, research labs, and institutes source critical reagents efficiently. This anti-MTRR polyclonal antibody supports research in methionine and folate metabolism, cobalamin processing, and related disease areas. Beyond individual antibodies, we supply validated antibody pairs, bulk ancillary reagents, optimized monoclonal/polyclonal preparations, and can assist in sourcing rare-target raw materials to streamline your development workflow.