Anti-MTPAP Rabbit Polyclonal Antibody for WB, ELISA - Q9NVV4

Core Product Specifications and Parameters

Biological Background: MTPAP Function and Localization

• MTPAP (Poly(A) RNA polymerase, mitochondrial), also known as PAPD1, SPAX4, TENT6, and mtPAP, is a mitochondrial polymerase responsible for synthesizing the 3' poly(A) tail of mitochondrial transcripts.
• In vitro, it can use all four nucleotides but shows higher activity with ATP and UTP, also functioning as a terminal uridylyltransferase (TUTase 1).
• MTPAP plays a role in replication-dependent histone mRNA degradation and may be involved in the terminal uridylation of mature histone mRNAs prior to degradation.
• It might be responsible for creating some UAA stop codons that are not encoded in mitochondrial DNA, contributing to mitochondrial gene expression.
• The protein localizes to both the cytoplasm and mitochondrion.
• MTPAP is ubiquitously expressed, with enhanced expression in tissues with high energy demands such as heart, brain, and skeletal muscle.
• Structural and biochemical studies highlight its nucleotide-binding and transferase activities, regulated by metal ions magnesium and manganese.
• Disease variants are associated with neurodegeneration, and the protein undergoes acetylation and alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 383-582 of human MTPAP. This central region may be conserved across species, so cross-reactivity with mouse is expected based on specification.
• For Western blot (WB), the predicted molecular weight is 66 kDa from UniProt, but consider potential post-translational modifications like acetylation that may shift mobility.
• This polyclonal antibody is listed for WB and ELISA; additional applications may require optimization.
• Perform appropriate controls, including knockout or siRNA knockdown, to confirm specificity in your experimental model.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-MTPAP polyclonal antibody, targeting a key mitochondrial RNA processing enzyme, exemplifies our commitment to supplying quality reagents for molecular biology research. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets to accelerate your assay development.