Anti-MTNR1A Rabbit Polyclonal Antibody (WB, IHC-P, ELISA) - P48039

Core Product Specifications and Parameters

Biological Background: MTNR1A Function and Localization

• MTNR1A encodes the melatonin receptor type 1A, also known as MT1 or MEL-1A-R, a high-affinity receptor for melatonin.
• It mediates the reproductive and circadian actions of melatonin, primarily through pertussis toxin-sensitive G proteins that inhibit adenylate cyclase activity.
• Possibly involved in sleep induction, where melatonin activation leads to the opening of the potassium channel KCNMA1/BK, decreasing synaptic transmission (By similarity).
• The receptor belongs to the G protein-coupled receptor (GPCR) superfamily and contains seven transmembrane domains, a disulfide bond, and glycosylation sites.
• MTNR1A localizes to the cell membrane.
• It is expressed in the hypophyseal pars tuberalis, hypothalamic suprachiasmatic nuclei (SCN), and hippocampus.
• Key structural features include 3D-structure determination, disulfide bond, and glycoprotein modifications.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 281–350), ensuring detection of the full-length receptor.
• For Western blot (WB), the predicted molecular weight is 39 kDa; consider using a suitable blocking agent and validate in your sample system.
• In immunohistochemistry (IHC-P), antigen retrieval may be necessary; optimize antibody dilution for your tissue type.
• The antibody cross-reacts with human, mouse, and rat, making it suitable for comparative studies across these species.
• For ELISA, confirm coating antigen concentration and antibody titer in preliminary experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering a comprehensive portfolio of validated antibodies, antibody pairs, and ancillary reagents for your assay development. For MTNR1A-related circadian rhythm and sleep research, we provide optimized polyclonal antibodies and can source rare-target raw materials to support your diagnostic and research needs. Contact us to explore custom sourcing solutions tailored to your project timeline.