Anti-MTNR1A Rabbit Polyclonal Antibody for WB and ELISA - P48039

Core Product Specifications and Parameters

Biological Background: MTNR1A Function and Localization

• MTNR1A encodes the melatonin receptor type 1A, a high-affinity receptor for melatonin, involved in regulating reproductive and circadian rhythms.
• The receptor functions via pertussis toxin-sensitive G proteins that inhibit adenylate cyclase activity, modulating intracellular cAMP levels.
• In the suprachiasmatic nucleus, melatonin activation of MTNR1A may contribute to sleep induction by promoting the opening of the potassium channel KCNMA1/BK and dissociating G-beta/gamma subunits, thus decreasing synaptic transmission.
• MTNR1A is a member of the G protein-coupled receptor (GPCR) superfamily, featuring seven transmembrane domains, a disulfide bond, and glycosylation sites.
• The receptor is localized to the cell membrane, consistent with its role in signal transduction.
• MTNR1A is predominantly expressed in the hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN), with additional expression in the hippocampus.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal peptide (aa 261-350); thus, the antibody recognizes an epitope in the intracellular domain. For western blot, validate band specificity using peptide blocking or positive controls expressing the target.
• Since this is a polyclonal preparation, batch-to-batch consistency may vary; consider re-evaluating each new lot.
• For ELISA, coating conditions and detection wavelength may require optimization; the immunogen peptide can serve as a control antigen.
• Cross-reactivity with rat is predicted; verify in rat tissues expressing MTNR1A.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, from monoclonal/polyclonal antibodies to bulk ancillary reagents. Whether you need validated antibody pairs for immunoassays or custom rare-target development, our platform streamlines your supply chain. This MTNR1A antibody supports research in circadian biology, sleep disorders, and melatonin receptor pathways.