Anti-MTMR6 Rabbit Polyclonal Antibody for WB, ELISA - Q9Y217

Core Product Specifications and Parameters

Biological Background: MTMR6 Function and Localization

• MTMR6 (Myotubularin-related protein 6) is a lipid phosphatase that specifically dephosphorylates phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) at the D-3 position, and binds with high affinity to these substrates. Related references: PMID:19038970 PMID:22647598
• It negatively regulates ER-Golgi protein transport.
• In association with MTMR9, MTMR6 participates in the late stages of macropinocytosis by dephosphorylating PtdIns(3)P in membrane ruffles. Related references: PMID:24591580
• MTMR6 acts as a negative regulator of the KCNN4/KCa3.1 potassium channel activity in CD4+ T-cells, likely by reducing intracellular levels of PtdIns(3)P. Related references: PMID:15831468
• It negatively regulates proliferation of reactivated CD4+ T-cells. Related references: PMID:16847315
• The formation of the MTMR6-MTMR9 complex stabilizes both protein levels and negatively regulates DNA damage-induced apoptosis. Related references: PMID:19038970 PMID:22647598
• Subcellular localization includes the cytoplasm, endoplasmic reticulum, ER-Golgi intermediate compartment, ruffle membrane, and perinuclear region.
• MTMR6 is expressed in CD4+ T-cells and is subject to alternative splicing and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 382-621 of human MTMR6, a region expected to be present in both full-length and alternatively spliced isoforms; consider validating in relevant cell lines.
• For Western blot, the predicted molecular weight is ~72 kDa; use whole-cell lysates from human, mouse, or rat T-cells or tissues expressing MTMR6.
• ELISA applications may be optimized for detecting endogenous or recombinant MTMR6; include appropriate positive and negative controls.
• Because MTMR6 localizes to membrane ruffles and intracellular compartments, consider fractionation or enrichment strategies to improve detection sensitivity in WB.
• Cross-reactivity with mouse and rat has been reported, but validate the antibody in your specific experimental model before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and researchers with streamlined access to validated IVD raw materials, including polyclonal and monoclonal antibodies, recombinant proteins, and ancillary reagents. For MTMR6—a key player in T-cell regulation and phosphoinositide signaling—we offer bulk supply and customized sourcing of antibodies and related reagents to accelerate your research or assay development. Leverage our one-stop sourcing platform to secure rare-target antibodies and complete your workflow efficiently.