Anti-MTMR4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NYA4

Core Product Specifications and Parameters

Biological Background: MTMR4 Function and Localization

• MTMR4 (myotubularin-related protein 4), also known as FYVE-DSP2 or ZFYVE11, belongs to the myotubularin family of dual-specificity phosphatases and contains a FYVE zinc finger domain.
• Acts as a lipid phosphatase that specifically dephosphorylates phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) at the D-3 position, generating PI and PI5P, respectively. Related references: PMID:11302699 PMID:16787938 PMID:20736309
• Negatively regulates endosomal dynamics by depleting PI3P on a subset of endosomal membranes, thereby controlling endocytic recycling and endosome maturation toward lysosomes. Related references: PMID:16787938 PMID:20736309 PMID:29962048
• Recruited to phagosome membranes and modulates phagocytosis and phagosome maturation through PI3P turnover. Related references: PMID:31543504
• Regulates innate immune responses by reducing PI3P levels in immune cells, dampening inflammatory signaling. Related references: PMID:30944173
• Functions as a molecular adapter during cytokinesis, facilitating midbody abscission independently of its phosphatase activity. Related references: PMID:25659891
• Negatively regulates TGF-beta and BMP signaling by dephosphorylating Smad proteins and promoting their retention in endosomes. Related references: PMID:20061380 PMID:23150675
• Subcellular localization includes early, recycling, and late endosomal membranes, as well as cytoplasmic vesicles and phagosome membranes.
• Broadly expressed across tissues, including brain, heart, kidney, spleen, liver, colon, testis, muscle, placenta, thyroid gland, pancreas, ovary, prostate, skin, peripheral blood, and bone marrow.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 920-1195) of human MTMR4; antibody reactivity may favor full-length protein or specific isoforms.
• For western blot (WB), the predicted molecular weight is ~133 kDa; consider using a gradient gel and appropriate transfer conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), expect endosomal and/or phagosomal staining; co-localization studies with compartment markers are recommended.
• The antibody recognizes human, mouse, and rat MTMR4 according to the supplier; independent validation in your specific model system is advised.
• For ELISA applications, optimal coating concentrations and detection conditions should be determined empirically.

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