Anti-MTMR2 Rabbit Polyclonal Antibody for WB, ELISA - Q13614

Core Product Specifications and Parameters

Biological Background: MTMR2 Function and Localization

• MTMR2 (Myotubularin-related protein 2) is a lipid phosphatase that specifically dephosphorylates phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) at the D-3 position, thereby generating phosphatidylinositol and phosphatidylinositol 5-phosphate. Related references: PMID:11733541 PMID:12668758 PMID:14690594
• It plays a critical role in autophagy, regulating both initiation and autophagosome maturation, as demonstrated by recent studies. Related references: PMID:35580604
• MTMR2 is a member of the myotubularin family of dual-specificity phosphatases, which are involved in phosphoinositide metabolism and membrane trafficking.
• The protein is localized to the cytoplasm, early endosome membranes, perinuclear regions, and cell projections such as axons, consistent with its role in neuronal function and membrane dynamics.
• Post-translational modifications including phosphorylation have been identified, and the protein contains a coiled-coil domain, possibly mediating protein-protein interactions.
• Mutations in the MTMR2 gene are associated with Charcot-Marie-Tooth disease type 4B1 (CMT4B1), a severe demyelinating neuropathy characterized by myelin outfolding and axonal loss.
• Alternative splicing of MTMR2 generates multiple transcript variants encoding distinct isoforms, which may have tissue-specific expression patterns.
• The protein is also identified as a proteomics identification reference and is involved in lipid metabolism pathways.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 404-643) of human MTMR2, which is selected to reduce cross-reactivity with other myotubularin family members; consider using this antibody in Western blotting and ELISA applications with appropriate positive controls.
• For Western blotting, the expected molecular weight is approximately 73 kDa. Optimize the dilution factor (e.g., 1:500–1:2000) for your specific experimental system, and validate specificity using knockout or siRNA-treated samples if available.
• Due to its cross-reactivity with mouse and rat, this antibody can be utilized for rodent model studies. Ensure proper sample preparation and loading controls are included.
• In ELISA, titrate the antibody and use a relevant peptide or protein standard to confirm binding specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined supply chain for IVD raw materials, technical services, and consulting. For researchers investigating phosphoinositide metabolism or Charcot-Marie-Tooth disease, we can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need rare-target raw materials or custom solutions, CamelBio supports your projects from concept to clinic.