Anti-MTMR14 Rabbit Polyclonal Antibody for WB, ELISA - Q8NCE2

Core Product Specifications and Parameters

Biological Background: MTMR14 Function and Localization

• Protein Identity: MTMR14 (Myotubularin-related protein 14), encoded by the MTMR14 gene (also known as C3orf29), is also referred to as HCV NS5A-transactivated protein 4 splice variant A-binding protein 1 (NS5ATP4ABP1), hJumpy, and phosphatidylinositol-3-phosphate phosphatase.
• Enzymatic Function: Functions as a lipid phosphatase that specifically dephosphorylates the D-3 position of phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2), generating phosphatidylinositol and phosphatidylinositol 5-phosphate, respectively.
• Subcellular Localization: Predominantly localizes to the cytoplasm.
• Tissue Expression: Expressed in a broad range of tissues, including heart, skeletal muscle, placenta, liver, lung, kidney, and pancreas.
• Post-Translational Modifications: Subject to acetylation, methylation, and phosphorylation; designated as a glycoprotein and phosphoprotein in UniProt.
• Disease Association: Associated with disease variants, as indicated by the UniProt keyword, suggesting potential involvement in human pathology.
• Isoform Diversity: Undergoes alternative splicing, yielding multiple protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment corresponding to the N-terminal 280 amino acids of human MTMR14 (NP_001070993.1), covering a region that likely includes conserved catalytic domains. This design may provide broad isoform recognition, but validation for specific splice variants is recommended.
• Given its cytoplasmic localization, MTMR14 can be detected in whole-cell lysates prepared using standard RIPA buffer. Consider enriching for membrane-associated fractions if studying its role at endosomal membranes.
• For Western blot analysis, a band corresponding to the predicted molecular weight of ~72 kDa should be observed; however, due to alternative splicing or post-translational modifications, additional bands may appear. Optimize blocking and antibody dilution conditions for your specific sample.
• The product is validated for ELISA; if developing a quantitative assay, we recommend titrating the antibody against a standard curve using recombinant MTMR14 protein.
• Cross-reactivity with human and mouse MTMR14 is confirmed, making it suitable for studies in common model systems. If extending to other species, verify sequence homology and perform pilot experiments.

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CamelBio partners with diagnostic manufacturers, laboratories, and research institutes to provide comprehensive access to IVD raw materials, facilitating development from concept to clinical application. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets. For inquiries regarding this MTMR14 rabbit polyclonal antibody—ideal for investigating phosphoinositide metabolism, neuromuscular disorders, or cellular signaling—our team stands ready to support your procurement and custom supply needs.