Anti-MTMR14 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8NCE2

Core Product Specifications and Parameters

Biological Background: MTMR14 Function and Localization

• MTMR14 (myotubularin-related protein 14), also known as hJumpy, NS5ATP4ABP1, or C3orf29, is a lipid phosphatase belonging to the myotubularin family.
• It specifically catalyzes the dephosphorylation of phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) at the D-3 position, generating phosphatidylinositol and PI5P, respectively.
• As a dual-specificity phosphatase, MTMR14 plays a critical role in phosphoinositide metabolism, regulating membrane trafficking, endosomal dynamics, and autophagy.
• Subcellular localization is predominantly cytoplasmic, consistent with its involvement in endosomal and autophagosomal pathways.
• MTMR14 is broadly expressed across human tissues, including heart, skeletal muscle, placenta, liver, lung, kidney, and pancreas.
• UniProt annotations identify multiple post-translational modifications (acetylation, methylation, phosphorylation), alternative splicing, and a glycoprotein nature, indicating complex regulatory control.
• UniProt annotates MTMR14 with a 'Disease variant' keyword, suggesting that genetic alterations are linked to human pathology.

Experimental Guidance and Technical Tips

• The immunogen comprises a recombinant fragment spanning amino acids 335–500 of human MTMR14, which is likely to capture multiple isoforms and provide broad reactivity.
• As a polyclonal antibody, it offers high sensitivity; however, optimization of blocking and washing conditions is recommended to minimize non‑specific signals.
• In Western blot, a major band near 72 kDa is expected; confirm specificity using knockout or knockdown controls.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols should be empirically tested due to the cytoplasmic localization of MTMR14.
• ELISA applications may require coating with the immunogen or recombinant antigen for optimal detection; assay linearity and matrix effects should be validated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, delivering this MTMR14 Rabbit pAb alongside a wide portfolio of validated antibodies, antigens, and ancillary reagents. Our integrated supply chain and technical support accelerate lipid phosphatase research and diagnostic assay development from concept to clinic. Contact us to discuss customized sourcing solutions for rare targets and bulk requirements.