Anti-MTMR1 Rabbit Polyclonal Antibody for WB and ELISA - Q13613

Core Product Specifications and Parameters

Biological Background: MTMR1 Function and Localization

• MTMR1, or myotubularin-related protein 1, is a lipid phosphatase that specifically dephosphorylates phosphatidylinositol 3-phosphate (PI3P) at the D-3 position, generating phosphatidylinositol. Related references: PMID:11733541, PMID:27018598
• It also exhibits catalytic activity toward phosphatidylinositol 3,5-bisphosphate, producing phosphatidylinositol 5-phosphate. Related references: PMID:27018598
• The protein belongs to the myotubularin-related family and contains a conserved phosphatase domain.
• MTMR1 localizes to the cell membrane and cytoplasm, consistent with its role in phosphoinositide metabolism.
• Key biological processes involving MTMR1 include lipid metabolism and phosphoinositide-mediated signaling.
• Post-translational modifications such as acetylation and phosphorylation have been identified through proteomics studies.
• Alternative splicing of the MTMR1 gene generates multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a central region (amino acids 300–400) of human MTMR1; consider this when assessing isoform specificity.
• For Western blotting, the expected protein molecular weight is approximately 75 kDa; use appropriate gel concentrations and transfer conditions.
• This antibody cross-reacts with human and mouse samples; validate in the relevant tissue or cell line before quantitative assays.
• For ELISA, optimize coating antigen concentration and detection antibody dilution to achieve optimal signal-to-noise ratio.
• Extract proteins with lysis buffers compatible with membrane and cytoplasmic proteins to ensure efficient detection of MTMR1.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research institutions, offering validated antibodies and ancillary reagents under one roof. This MTMR1 polyclonal antibody supports investigations into lipid metabolism and phosphoinositide signaling, and is part of our growing catalog of research-use raw materials. We also provide customized sourcing for rare targets and bulk supply options to streamline your assay development pipeline.