Anti-MTIF3 Polyclonal Antibody for WB, ELISA - Q9H2K0

Core Product Specifications and Parameters

Biological Background: MTIF3 Function and Localization

• MTIF3 encodes mitochondrial translation initiation factor 3 (IF-3mt), a key regulator of mitochondrial protein synthesis.
• IF-3mt binds specifically to the 28S small ribosomal subunit within mitochondria.
• This binding shifts the equilibrium between 55S monosomes and their component 39S and 28S subunits, favoring subunit dissociation.
• By promoting free 28S subunits, IF-3mt enhances the availability of small ribosomal subunits for translation initiation.
• The protein is localized exclusively to the mitochondrion, consistent with its role in organellar translation.
• MTIF3 is characterized as an initiation factor within the broader protein biosynthesis machinery, and its structure has been solved (3D-structure evidence).
• An N-terminal transit peptide targets the precursor protein to mitochondria, where it is processed to the mature form.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human MTIF3 (aa 1-278), so the polyclonal antibody may recognize multiple epitopes; consider running a blocking peptide control if non-specific bands appear in WB.
• For WB, the predicted molecular weight of MTIF3 is approximately 32 kDa; a mitochondrial-enriched lysate can be used to enhance signal detection.
• In ELISA, the antibody can be employed as a capture or detection reagent; validate assay conditions using recombinant MTIF3 protein or cell lysates from human or rat samples.
• Cross-reactivity with rat has been confirmed; for other species, perform in silico sequence alignment and experimental verification before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, labs, and research institutes with high-quality IVD raw materials, including validated antibody pairs and polyclonal antibodies like this anti-MTIF3. Targeting mitochondrial translation research, we provide one-stop access to bulk ancillary reagents, recombinant proteins, and rare target sourcing. Whether you need optimized antibodies for WB/ELISA development or full-scale procurement support, our supply network ensures reliable, cost-effective solutions to accelerate your project from concept to clinic.