Anti-MTIF3 Polyclonal Antibody for WB, ELISA - Q9H2K0

Core Product Specifications and Parameters

Biological Background: MTIF3 Function and Localization

• MTIF3 encodes mitochondrial translation initiation factor 3 (IF-3mt), a key factor in mitochondrial protein synthesis.
• IF-3mt binds to the 28S small ribosomal subunit, promoting dissociation of 55S ribosomes into 39S and 28S subunits, thus enhancing availability of 28S subunits for translation initiation.
• The protein is localized in the mitochondrion, consistent with its role in mitochondrial translation.
• MTIF3 contains an N-terminal transit peptide that targets the protein to mitochondria, where it is processed to its mature form.
• It belongs to the translation initiation factor family and is essential for initiating protein biosynthesis in mitochondria.
• MTIF3 is a reference proteome protein and has been identified through proteomics, with a molecular weight of approximately 32 kDa (predicted 31.7 kDa).
• Keywords: 3D-structure, direct protein sequencing, initiation factor, mitochondrion, protein biosynthesis, reference proteome, transit peptide.

Experimental Guidance and Technical Tips

• The polyclonal antibody was raised against a recombinant fusion protein covering amino acids 1-278, which likely represents nearly full-length MTIF3. It may recognize both linear and conformational epitopes.
• For Western blotting, consider using mitochondrial-enriched fractions or whole-cell lysates; a band around 32 kDa is expected. Validate detection in your specific sample system.
• Due to sequence homology, cross-reactivity with mouse and rat MTIF3 is reported; confirm performance in your species of interest.
• For ELISA, perform standard antibody titration to optimize signal-to-noise ratio; use non-immune rabbit IgG as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with integrated access to IVD raw materials, including validated antibodies like this anti-MTIF3 polyclonal antibody for mitochondrial translation studies. Our one-stop supply chain includes antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing to support your assay development from concept to clinic.