Anti-MTHFR Monoclonal Antibody for WB, IF/ICC, ELISA - P42898

Core Product Specifications and Parameters

Biological Background: MTHFR Function and Localization

• MTHFR (Methylenetetrahydrofolate reductase (NADPH)) is a flavoprotein that catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a key step in homocysteine remethylation to methionine. Related references: PMID:29891918
• This enzymatic reaction represents a critical regulatory link between the folate cycle and the methionine cycle, essential for one-carbon metabolism.
• MTHFR is an allosteric enzyme that utilizes FAD as a cofactor and NADP as a coenzyme, categorized as an oxidoreductase.
• The MTHFR gene undergoes alternative splicing, producing multiple transcript variants, and the protein is known to be phosphorylated at specific residues.
• Genetic variations in MTHFR are associated with susceptibility to various diseases, including neural tube defects, cardiovascular disorders, and schizophrenia.
• The protein has a molecular weight of approximately 75 kDa and belongs to the reference proteome for proteomics identification.
• Three-dimensional structural studies have provided insights into its domain organization and allosteric regulation by S-adenosylmethionine.

Experimental Guidance and Technical Tips

• The immunogen for this antibody covers amino acids 363-656 of human MTHFR, likely targeting the C-terminal regulatory domain. Verify epitope accessibility in your specific application.
• For Western blot (WB), consider using standard loading controls and optimize antibody dilution based on endogenous expression levels in your cell or tissue lysates.
• In immunofluorescence (IF/ICC), titrate the antibody to achieve specific staining, and include appropriate negative controls to confirm signal specificity.
• For ELISA applications, validate the antibody's binding affinity and detection range with recombinant MTHFR protein or standards.
• Given the cross-reactivity with mouse and rat, this antibody is suitable for comparative studies, but validate in the relevant species-derived samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers IVD manufacturers and research teams by providing comprehensive access to high-quality raw materials, including validated antibody pairs, monoclonal antibodies, and ancillary reagents. Specializing in rare and critical targets like MTHFR, we support your one-carbon metabolism, folate pathway, and biomarker assay development needs. From concept to clinic, count on CamelBio as your reliable sourcing partner for bulk and custom raw material procurement.