Anti-MTHFD2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P13995

Core Product Specifications and Parameters

Biological Background: MTHFD2 Function and Localization

• MTHFD2 (methylenetetrahydrofolate dehydrogenase 2), also known as NMDMC, is a bifunctional enzyme containing NAD-dependent dehydrogenase and cyclohydrolase activities, encoded by the MTHFD2 gene.
• It plays a critical role in one-carbon metabolism, catalyzing the interconversion of tetrahydrofolate derivatives within the mitochondrion.
• The enzyme shows a preference for NAD as a cofactor but can utilize NADP at reduced efficiency, as indicated by UniProt functional annotation.
• Subcellularly, MTHFD2 is localized to the mitochondrial matrix, consistent with its involvement in mitochondrial folate metabolism.
• Post-translational modifications include acetylation and ubiquitin conjugation (isopeptide bond), which may regulate enzyme activity or stability.
• The protein undergoes alternative splicing, potentially generating isoforms with distinct properties; the immunogen covers a region common to the canonical sequence.
• With a calculated molecular weight of approximately 37.9 kDa (observed at ~38 kDa), MTHFD2 is classified under oxidoreductase and hydrolase protein families.
• Keywords such as transit peptide confirm mitochondrial import, and proteomics identification supports its detection in mass spectrometry-based studies.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning amino acids 40–250, which may detect full-length and truncated variants; validate band patterns in your sample system.
• For western blotting, a band around 38 kDa is expected; consider using a positive control lysate from MTHFD2-expressing tissues (e.g., liver or kidney).
• In IHC-P, optimize antigen retrieval and use a known positive tissue section; because MTHFD2 is mitochondrial, staining may appear punctate.
• For IF/ICC, fixation and permeabilization conditions should preserve mitochondrial integrity; co-staining with a mitochondrial marker is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; pre-adsorption against the immunogen may reduce background if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. For MTHFD2-related projects—whether studying one-carbon metabolism, mitochondrial dysfunction, or developing diagnostic assays—CamelBio serves as a reliable procurement partner. Our team also supports rare-target sourcing and technical consultation to accelerate your transition from concept to clinic.