Anti-MTHFD1L Polyclonal Antibody for WB, IHC-P, ELISA - Q6UB35

Core Product Specifications and Parameters

Biological Background: MTHFD1L Function and Localization

• MTHFD1L encodes monofunctional C1-tetrahydrofolate synthase, a mitochondrial enzyme also known as formyltetrahydrofolate synthetase.
• It is proposed to bridge mitochondrial and cytoplasmic one-carbon metabolism by complementing the enzymatic activities of MTHFD2.
• The protein localizes to the mitochondrion, consistent with its role in folate-mediated one-carbon transfer.
• MTHFD1L is expressed in most tissues, with highest levels in placenta, thymus, and brain; lower expression in liver and skeletal muscle.
• The gene undergoes alternative splicing and the protein is subject to acetylation and phosphorylation.
• Upregulation of MTHFD1L in colon adenocarcinoma suggests a potential link to cancer cell metabolism.
• The protein contains ATP-binding and nucleotide-binding domains and functions as a ligase in one-carbon metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 759-978), which is unique to MTHFD1L; confirm specificity by using appropriate positive and negative controls.
• For Western blot (WB), a band around 106 kDa is expected; consider testing a range of antibody dilutions and validate with lysates from human, mouse, or rat tissues/cells.
• In immunohistochemistry (IHC-P), antigen retrieval methods may be required; optimize staining conditions on known positive tissues (e.g., placenta, brain).
• For ELISA, the antibody can potentially serve as capture or detection reagent; pair with another MTHFD1L-specific antibody or use recombinant protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. This anti-MTHFD1L polyclonal antibody supports research into one-carbon metabolism and cancer biology. Our supply chain expertise also covers rare target raw-material procurement and technical consultation, bridging the gap from concept to clinic.