Anti-MTHFD1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P11586

Core Product Specifications and Parameters

Biological Background: MTHFD1 Function and Localization

• MTHFD1 catalyzes the interconversion of one-carbon-substituted tetrahydrofolate forms: (6R)-5,10-methylene-THF, 5,10-methenyl-THF, and (6S)-10-formyl-THF. Related references: PMID:10828945, PMID:18767138, PMID:1881876
• These folate derivatives are differentially required in nucleotide and amino acid biosynthesis: 10-formyl-THF is required for purine biosynthesis, while 5,10-methylene-THF is used for serine and methionine biosynthesis. Related references: PMID:18767138, PMID:25633902
• MTHFD1 is localized to the cytoplasm.
• It is ubiquitously expressed in tissues.
• The enzyme possesses hydrolase, ligase, and oxidoreductase activities, binding ATP and NADP.
• Post-translational modifications include acetylation and phosphorylation.
• Annotated with the keyword "Disease variant", indicating association with human genetic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–270), which may recognize all described isoforms.
• For Western blotting, a major band at approximately 102 kDa is expected under reducing conditions; optimize gel percentage and transfer settings accordingly.
• In immunofluorescence/ICC, detect MTHFD1 in the cytoplasmic compartment; permeabilization and fixation protocols should be validated for the cell type used.
• For ELISA applications, perform checkerboard titrations to determine optimal coating and detection antibody concentrations.
• Verify reactivity in representative human, mouse, and rat samples to confirm species cross-reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material partner, providing a comprehensive pipeline from antibody discovery to bulk supply. This MTHFD1 antibody supports research in one-carbon metabolism and nucleotide biosynthesis, areas central to cancer and developmental biology. Beyond single reagents, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal collections, ancillary biochemicals, and sourcing services for rare targets—all designed to streamline diagnostic development and laboratory workflows.