Anti-MTH1 Rabbit Monoclonal Antibody for WB, ELISA - P36639

Core Product Specifications and Parameters

Biological Background: MTH1 Function and Localization

• Oxidized purine nucleoside triphosphate hydrolase, also known as MTH1, NUDT1, 8-oxo-dGTPase, and Nucleoside diphosphate-linked moiety X motif 1, is a sanitizer of the oxidized nucleotide pool. Related references: PMID:10608900, PMID:12857738, PMID:22556419
• Catalyzes hydrolysis of 2-oxo-dATP, 2-oxo-ATP, 8-oxo-dGTP, and 8-oxo-dATP to prevent incorporation into DNA and subsequent A:T to C:G and G:C to T:A transversions. Related references: PMID:10373420, PMID:11139615, PMID:10608900, PMID:11756418
• Also hydrolyzes methylated purine nucleoside triphosphates, providing antimutagenic activity that protects cells from oxidative stress. Related references: PMID:30304478, PMID:32144205, PMID:10608900, PMID:24695224
• Belongs to the Nudix hydrolase family, containing a Nudix motif 1; requires magnesium as a cofactor and may bind RNA.
• Localizes to the cytoplasm, cytosol, mitochondrial matrix, and nucleus; widely expressed with highest levels in thymus, testis, embryo, and proliferating blood lymphocytes.
• Molecular weight is approximately 18 kDa (UniProt: 17952 Da); alternative initiation generates isoforms.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 50–200 of human MTH1 (P36639), which may be suitable for detecting both cytoplasmic and mitochondrial pools of the enzyme.
• For western blot (WB), the predicted molecular weight is approximately 18 kDa; consider using a reducing gel and loading appropriate controls from human cell lysates known to express MTH1 (e.g., thymus, testis).
• For ELISA, validate the antibody’s performance with recombinant MTH1 protein or native protein in human sample matrices.
• Since MTH1 is widely expressed, background may vary; optimize blocking and antibody dilution for each sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs a streamlined source for validated IVD raw materials. This anti-MTH1 rabbit monoclonal antibody is one component within our extensive portfolio of antibodies targeting nucleotide metabolism and oxidative stress markers. Beyond single reagents, we offer matched antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to support your assay development from concept to clinic.