Anti-MTH1 Polyclonal Antibody for WB, ELISA - P36639

Core Product Specifications and Parameters

Biological Background: MTH1 Function and Localization

• MTH1, also known as NUDT1 (Nudix motif 1), is a sanitizer of the oxidized nucleotide pool, hydrolyzing oxidized purine nucleoside triphosphates to prevent DNA damage. Related references: PMID:10608900, PMID:12857738, PMID:22556419
• It catalyzes the hydrolysis of damaged nucleotides such as 2-oxo-dATP, 2-oxo-ATP, 8-oxo-dGTP, and 8-oxo-dATP, thereby preventing their misincorporation into DNA. Related references: PMID:10373420, PMID:11139615
• Through this activity, MTH1 prevents A:T to C:G and G:C to T:A transversions, acting as a critical antimutagenic enzyme in the defense against oxidative DNA damage. Related references: PMID:10373420, PMID:10608900, PMID:11756418
• MTH1 also hydrolyzes methylated purine nucleoside triphosphates, further protecting the genome from alkylation-induced mutations. Related references: PMID:30304478, PMID:32144205
• The enzyme is localized in the cytoplasm, cytosol, mitochondrial matrix, and nucleus, aligning with its role in safeguarding both nuclear and mitochondrial genomes.
• MTH1 is widely expressed, with the highest levels found in thymus, testis, embryo, and proliferating blood lymphocytes, indicating importance in tissues with high replication rates.
• The protein possesses a Nudix hydrolase domain, requires magnesium for activity, and has a molecular weight of approximately 18 kDa. Three-dimensional structures are available, and alternative initiation variants exist.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-179 of human MTH1, allowing recognition of the full-length protein. For Western blot, a band of approximately 18 kDa is expected; validate with a positive control lysate from human cell lines.
• For ELISA, consider using the recombinant MTH1 protein as a coating antigen and testing a dilution series of the antibody to establish optimal working concentrations.
• This antibody is reported to react with human MTH1; cross-reactivity with other species has not been tested. If using in other species, verify sequence homology and validate experimentally.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated IVD raw-material partner, CamelBio provides reliable sourcing for high-quality antibodies like this anti-MTH1 polyclonal. Our comprehensive portfolio supports diagnostic development in areas such as oxidative stress, DNA damage repair, and cancer biomarker research. From validated antibody pairs and bulk ancillary reagents to rare target sourcing, we streamline your supply chain from concept to clinic.