Anti-MTFMT Rabbit Polyclonal Antibody for WB, ELISA - Q96DP5

Core Product Specifications and Parameters

Biological Background: MTFMT Function and Localization

• MTFMT encodes a mitochondrial methionyl-tRNA formyltransferase essential for initiating mitochondrial translation.
• The enzyme catalyzes the formylation of methionyl-tRNA(Met) to fMet-tRNA(Met), a prerequisite for translation initiation in mitochondria.
• Located in the mitochondrion, consistent with its role in mitochondrial protein synthesis.
• Alternative splicing generates multiple transcript variants (as indicated by keywords), which may contribute to functional diversity.
• Genetic mutations in MTFMT are associated with mitochondrial diseases (COXPD15, MC1DN27), highlighting its clinical significance.
• Belongs to the formyltransferase family; conserved across species.
• Keywords: Transferase, Protein biosynthesis, Mitochondrion, Primary mitochondrial disease.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 180-389 of human MTFMT; verify that the antibody recognizes endogenous protein at the expected ~44 kDa band in mitochondrial lysates.
• For Western blot, load appropriate controls and consider using knockout or knockdown validation to confirm specificity.
• In ELISA, titrate the antibody under standardized conditions and include relevant blanks to minimize background.
• This polyclonal antibody may exhibit lot-to-lot variability; include positive and negative controls in each experiment.
• Cross-reactivity with mouse and rat orthologs is reported; validate in the sample matrix of interest before quantitative studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research institutions with a robust pipeline of IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies. For projects targeting mitochondrial disorders or protein synthesis, this MTFMT antibody is available alongside bulk ancillary reagents and custom sourcing services to accelerate your assay development from concept to clinic.