Anti-MTERFD1 Rabbit Polyclonal Antibody for WB, ELISA - Q96E29

Core Product Specifications and Parameters

Biological Background: MTERFD1 Function and Localization

• MTERFD1 (also known as mTERF3) is a mitochondrial transcription termination factor encoded by the MTERF3 gene.
• Binds promoter DNA and regulates initiation of mitochondrial transcription. Related references: PMID:17662942
• Required for normal mitochondrial transcription, translation, and assembly of respiratory complexes (by similarity).
• Maintains 16S rRNA levels and functions in mitochondrial ribosome assembly by regulating biogenesis of the 39S ribosomal subunit (by similarity).
• Alternative splicing produces multiple protein isoforms.
• Localizes to the mitochondrion.
• Highly expressed in heart, liver, kidney, and testis; lower expression in brain, spleen, and lung.
• Contains a DNA-binding domain and a transit peptide for mitochondrial import.

Experimental Guidance and Technical Tips

• For Western blot, consider using mitochondrial-enriched lysates to enhance detection of MTERFD1, given its mitochondrial localization.
• Validate the antibody in human and mouse sample systems to confirm cross-reactivity and specificity.
• Optimize antibody dilution for each application (WB/ELISA) by performing a titration series.
• The immunogen spans amino acids 69–300, which excludes the N-terminal transit peptide; this may allow detection of both precursor and mature protein forms.
• Confirm band size at approximately 48 kDa; additional bands may represent isoforms from alternative splicing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-MTERFD1 rabbit pAb. Whether you need bulk ancillary reagents or assistance sourcing rare-target antibodies for mitochondrial transcription studies, CamelBio's integrated supply chain and technical support bridge the gap from concept to clinic.