Anti-MTCO2 Rabbit Monoclonal Antibody for WB, IF, IHC-P, ELISA - P00403

Core Product Specifications and Parameters

Biological Background: MTCO2 Function and Localization

• MTCO2, also known as cytochrome c oxidase subunit 2 (COX2), is a core component of complex IV (cytochrome c oxidase) of the mitochondrial respiratory chain.
• It plays a critical role in the electron transport chain, accepting electrons from reduced cytochrome c at its dinuclear copper A center and transferring them to the catalytic site in subunit 1 for oxygen reduction to water.
• This process drives oxidative phosphorylation, creating a proton gradient across the inner mitochondrial membrane that fuels ATP synthesis.
• Localized to the mitochondrial inner membrane, MTCO2 is an integral transmembrane protein with its copper center facing the intermembrane space.
• It contains a copper ion-binding site and is essential for cytochrome c oxidase assembly and activity; mutations in MTCO2 are associated with mitochondrial diseases.
• The protein is encoded by the mitochondrial genome (MT-CO2 gene) and is synthesized with a cytochrome c-type biogenesis motif.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 128–227 of human MTCO2, a region likely exposed and conserved; for WB, expect a band at approximately 26 kDa.
• For immunofluorescence (IF/ICC) or immunohistochemistry (IHC-P), mitochondrial staining should be observed; consider co-staining with a mitochondrial marker to confirm localization.
• ELISA and WB may be used to detect MTCO2 in human cell lysates or purified mitochondrial fractions; validate in the relevant sample system and include appropriate positive and negative controls.
• Optimal working dilutions should be determined empirically; cross-reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive procurement platform for IVD raw materials, including high-performance antibodies like this MTCO2 rabbit monoclonal antibody, ideal for mitochondrial research and oxidative phosphorylation studies. Our one-stop sourcing service covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your development from concept to clinic.