Anti-MTCO2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00403

Core Product Specifications and Parameters

Biological Background: Cytochrome c oxidase subunit 2 Function and Localization

• MT-CO2 encodes cytochrome c oxidase subunit 2 (also known as COX2 or COII), a core component of the mitochondrial respiratory chain complex IV.
• As part of cytochrome c oxidase, it catalyzes the final step of the electron transport chain: the reduction of molecular oxygen to water, coupled to proton pumping across the inner mitochondrial membrane.
• Subunit 2 contains a dinuclear copper A (CuA) center that accepts electrons from reduced cytochrome c in the intermembrane space and transfers them to heme A in subunit 1.
• The protein is an integral membrane protein located in the mitochondrial inner membrane, with multiple transmembrane helices.
• It is encoded by the mitochondrial genome (MT-CO2) and is essential for oxidative phosphorylation and ATP production.
• Mutations in MT-CO2 are associated with primary mitochondrial diseases, affecting tissues with high energy demands.

Experimental Guidance and Technical Tips

• For Western blot, the predicted protein weight is approximately 26 kDa; mitochondrial enrichment may improve detection sensitivity.
• The immunogen corresponds to a conserved region (amino acids 40-100) of human MTCO2, which is highly homologous in mouse and rat, supporting the reported cross-reactivity.
• In IHC-P and IF/ICC, validate staining in tissues/cells with abundant mitochondria; consider using mitochondrial markers as co-stains.
• For ELISA applications, confirm specificity by peptide competition or by testing against recombinant MTCO2 protein.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio specializes in supplying high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for diagnostic development.
• This MTCO2 antibody targets a key mitochondrial complex IV subunit, relevant for mitochondrial disease research and diagnostic assay development.
• Whether you need rare target raw-material sourcing or comprehensive technical consultation from concept to clinic, CamelBio offers a seamless procurement experience.