Anti-MTCO2 (COX2) Rabbit Monoclonal Antibody for WB - P00403

Core Product Specifications and Parameters

Biological Background: MTCO2 (COX2) Function and Localization

• MTCO2 (COX2) encodes cytochrome c oxidase subunit 2, a core component of mitochondrial respiratory chain complex IV.
• Complex IV catalyzes the terminal step of oxidative phosphorylation, reducing molecular oxygen to water using electrons from cytochrome c and protons from the matrix.
• The protein contains a dinuclear copper A (CuA) center that accepts electrons from cytochrome c and transfers them to the catalytic binuclear center in subunit 1.
• MTCO2 is located in the mitochondrial inner membrane, spanning the membrane with transmembrane helices.
• Post-translational modifications include cytochrome c-type biogenesis, critical for its redox function.
• Genetic variants of MTCO2 have been linked to primary mitochondrial disorders.
• MTCO2 acts as a translocase, participating in proton translocation across the inner mitochondrial membrane to generate the electrochemical gradient.
• The mature protein has a molecular weight of approximately 26 kDa (predicted 25.6 kDa).

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 101–200 of mouse MTCO2) is highly conserved across human, mouse, and rat, supporting observed cross-reactivity; use appropriate species-specific controls.
• For Western blotting, expect a band around 26 kDa and validate protein loading with a mitochondrial marker.
• In immunofluorescence or immunohistochemistry, confirm mitochondrial localization with organelle-specific dyes.
• For ELISA, optimize coating conditions and test whether the antibody recognizes the native or recombinant protein in your sample system.
• As a rabbit monoclonal antibody, use anti-rabbit secondary antibodies for detection.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing platform, CamelBio connects diagnostic manufacturers, laboratories, and research institutes with validated antibodies and ancillary reagents. For your mitochondrial and metabolic disease research, we also offer bulk-ordering options, alternative clone sourcing, and matched antibody pairs. Contact us for customized procurement solutions that accelerate your assay development.