Anti-MTCO1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00395

Core Product Specifications and Parameters

Biological Background: MTCO1 Function and Localization

• MTCO1 encodes Cytochrome c oxidase subunit 1 (COX1), the catalytic core subunit of mitochondrial respiratory chain complex IV.
• It is a multi-pass transmembrane protein localized to the mitochondrial inner membrane, harboring essential redox-active metal centers.
• The enzyme catalyzes the terminal reduction of molecular oxygen to water, utilizing electrons from cytochrome c and protons from the matrix, thereby contributing to the proton gradient essential for ATP synthesis.
• The functional unit includes a binuclear center (BNC) formed by heme A3 and copper B (CuB), together with a dinuclear copper A center (CuA) in subunit 2 and heme A.
• Mutations in MTCO1 are implicated in Leber hereditary optic neuropathy (LHON) and non-syndromic deafness, underscoring its role in human mitochondrial pathophysiology.
• The protein requires binding of calcium, magnesium, and sodium ions for structural stability and catalytic efficiency.
• As a mitochondrial DNA-encoded protein, its expression is regulated by nuclear-encoded factors and post-translational mechanisms.
• Keywords from UniProt: 3D-structure, Calcium, Copper, Deafness, Disease variant, Electron transport, Heme, Iron, Leber hereditary optic neuropathy, Magnesium, Membrane, Metal-binding, Mitochondrion, Mitochondrion inner membrane, Non-syndromic deafness, Primary mitochondrial disease, Proteomics identification, Reference proteome, Respiratory chain, Sodium, Translocase, Transmembrane, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 400–500) of human MTCO1, suggesting the antibody may target an epitope in this chemically distinct domain; verify specificity in your experimental system.
• For Western blot, a specific band near 57 kDa is anticipated; include appropriate positive and negative controls (e.g., mitochondrial fractions, siRNA knockdown) to confirm target identity.
• In IHC-P, expect mitochondrial staining patterns; optimize antigen retrieval and consider using mitochondrial markers for co-localization studies.
• For IF/ICC, permeabilization is necessary due to the transmembrane localization; validate subcellular distribution.
• ELISA applications may require coating with the immunogen peptide or full-length protein; standardize conditions to achieve optimal sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials, connecting diagnostic manufacturers and research labs with high-quality antibodies and ancillary reagents. This anti-MTCO1 polyclonal antibody serves as a valuable tool for investigating mitochondrial function, oxidative phosphorylation disorders, and related disease mechanisms. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and facilitate sourcing of rare-target raw materials to accelerate your diagnostic development pipeline.