Anti-MTCO1 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P00395

Core Product Specifications and Parameters

Biological Background: MTCO1 Function and Localization

• MTCO1 (cytochrome c oxidase subunit 1) is a core component of complex IV (cytochrome c oxidase) of the mitochondrial respiratory chain, which catalyzes the final step of electron transport: the reduction of molecular oxygen to water.
• The enzyme transfers electrons from reduced cytochrome c in the intermembrane space via the dinuclear copper A center of subunit 2 and heme A of subunit 1 to the binuclear center (heme A3 and copper B) in subunit 1, where oxygen is reduced using four protons from the mitochondrial matrix.
• This process contributes to the generation of an electrochemical proton gradient across the inner mitochondrial membrane, which drives ATP synthesis.
• MTCO1 is encoded by the mitochondrial genome (gene MT-CO1) and is a highly conserved transmembrane protein embedded in the inner mitochondrial membrane.
• It contains redox-active metal centers including heme A, heme A3, copper ions, and a magnesium ion; calcium and sodium ions also play structural roles.
• Mutations in MTCO1 are associated with human diseases, including Leber hereditary optic neuropathy (LHON) and non-syndromic deafness, underscoring its essential role in mitochondrial function.
• Keywords: 3D-structure, Calcium, Copper, Deafness, Disease variant, Electron transport, Heme, Iron, Leber hereditary optic neuropathy, Magnesium, Membrane, Metal-binding, Mitochondrion, Mitochondrion inner membrane, Non-syndromic deafness, Primary mitochondrial disease, Proteomics identification, Reference proteome, Respiratory chain, Sodium, Translocase, Transmembrane, Transmembrane helix, Transport.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 479–513 of human MTCO1, a region that may be exposed on the matrix side of the inner mitochondrial membrane; consider this epitope location when designing detection and localization assays.
• For Western blot (WB), the predicted molecular weight is approximately 57 kDa; careful sample preparation and denaturing conditions are recommended to ensure proper electrophoresis of this hydrophobic membrane protein.
• In immunohistochemistry (paraffin, IHC-P), adequate antigen retrieval methods may be necessary; validate the staining pattern in relevant human tissue samples, particularly those rich in mitochondria.
• For ELISA applications, pairing with a suitable detection antibody and optimizing coating conditions should be performed using recombinant or native MTCO1 as standard.
• As with any research antibody, verify specificity and performance in your particular experimental model system before proceeding to critical studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials and technical services. This anti-MTCO1 monoclonal antibody is well-suited for studies of mitochondrial function, metabolic disorders, and related diseases. Leverage our capabilities in validated antibody pairs, bulk reagent supply, and rare target sourcing to accelerate your assay development from concept to clinic.