Anti-MTCH1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9NZJ7

Core Product Specifications and Parameters

Biological Background: MTCH1 Function and Localization

• MTCH1 (Mitochondrial carrier homolog 1), also known as Presenilin-associated protein (PSAP), is encoded by the MTCH1 gene (GeneID: 23787). It belongs to the mitochondrial carrier family but functions as an insertase rather than a transporter, with alternative names including PIG60, CGI-64, and SLC25A49.
• MTCH1 serves as a protein insertase that mediates the insertion of transmembrane proteins into the mitochondrial outer membrane. It specifically catalyzes the insertion of proteins with alpha-helical transmembrane regions—such as signal-anchored, tail-anchored, and multi-pass membrane proteins—but does not insert beta-barrel proteins. Related references: PMID:36264797
• Beyond its insertase activity, MTCH1 may also play a role in apoptosis, potentially via interactions with mitochondrial apoptotic pathways. Related references: PMID:12377771
• The protein localizes specifically to the mitochondrial outer membrane, anchoring its function and interactions.
• MTCH1 is widely expressed across tissues, with a predominant expression level in the brain, suggesting a specialized role in neuronal mitochondrial biology.
• Post‑translational modifications include alternative splicing and methylation, as indicated by proteomics data.
• Functional keywords associated with MTCH1 include membrane, mitochondrion, transport (as a carrier homolog), alternative splicing, and apoptosis, highlighting its diverse cellular roles.

Experimental Guidance and Technical Tips

• The immunogen for this antibody covers amino acids 100–250 of human MTCH1 (isoform NP_001258570.1), a region rich in soluble domains. This design favors the generation of high-titer polyclonal antibodies.
• For western blotting, the expected molecular weight of MTCH1 is approximately 42 kDa. Validate a single band in human cell lysates such as HEK293 or HeLa. Given the antibody’s cross-reactivity with mouse and rat, brain tissue lysates from these species can serve as additional positive controls.
• In immunohistochemistry on paraffin‑embedded sections, MTCH1 staining is expected to be mitochondrial and outer membrane‑associated. Optimize antigen retrieval and consider brain tissue sections as a robust positive control; co‑staining with mitochondrial markers is advised.
• For ELISA applications, the polyclonal nature of this antibody makes it suitable as either a capture or detection reagent. Titration of coating and blocking conditions is recommended to maximize signal-to-noise ratios.
• As with all polyclonal antibodies, validate performance in the relevant sample system and application before scaling assays to ensure reproducible results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research institutes, and laboratories with streamlined access to high‑quality IVD raw materials. This anti-MTCH1 polyclonal antibody supports investigations into mitochondrial biology, apoptosis, and neuronal function. As a one‑stop sourcing bridge, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets, helping you accelerate the journey from concept to clinic.