Anti-MTAP Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q13126

Core Product Specifications and Parameters

Biological Background: MTAP Function and Localization

• Catalyzes the phosphorolytic cleavage of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate, a crucial step in the methionine salvage pathway and polyamine breakdown; exhibits broad substrate specificity for 6-aminopurine nucleosides. Related references: PMID:3091600 PMID:8687427 PMID:10404592

• Also known as 5'-methylthioadenosine phosphorylase (MTAPase) and MTA phosphorylase.

• Encoded by the MTAP gene (MSAP) and classified within the purine nucleoside phosphorylase family.

• Localizes to both the cytoplasm and nucleus, as indicated by UniProt annotations.

• Ubiquitously expressed across human tissues, supporting its housekeeping role in polyamine metabolism.

• Undergoes acetylation and alternative splicing; additional keywords include 3D-structure, glycosyltransferase, and transferase.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human MTAP (aa 1-283), suggesting the antibody may recognize native and recombinant MTAP across multiple species (human, mouse, rat) where the epitope is conserved.
• For Western blot (WB), a band at ~31 kDa is expected; consider using appropriate protein extraction and loading controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and antibody dilution, as MTAP is present in both nuclear and cytoplasmic compartments.
• ELISA can be used for quantitative detection; validate performance with recombinant protein standards.
• Given MTAP's role in cancer and methionine metabolism, consider pairing with other pathway markers in multiplex assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This MTAP polyclonal antibody supports research into polyamine metabolism and purine salvage pathways, with applications in oncology and metabolic disease. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and tailored sourcing solutions for rare targets to streamline your assay development.