Anti-MTAP Polyclonal Antibody for WB, IF/ICC, ELISA - Q13126

Core Product Specifications and Parameters

Biological Background: MTAP Function and Localization

• Catalyzes the phosphorolytic cleavage of S-methyl-5′-thioadenosine (MTA) to adenine and 5-methylthioribose1-phosphate, a key reaction in polyamine metabolism.
  Related references: PMID:3091600 PMID:8687427 PMID:10404592
• Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis, thereby linking polyamine and methionine pathways.
  Related references: PMID:3091600 PMID:8687427 PMID:10404592
• Responsible for the first step in the methionine salvage pathway after MTA is generated from S-adenosylmethionine, recycling sulfur-containing metabolites.
  Related references: PMID:3091600 PMID:8687427 PMID:10404592
• Exhibits broad substrate specificity with a preference for 6-aminopurine nucleosides, highlighting its role in purine salvage.
  Related references: PMID:10404592
• Localizes to both the cytoplasm and nucleus, suggesting compartment-specific regulatory functions.
• Ubiquitously expressed across human tissues, indicating a housekeeping role in cellular metabolism.
• The protein is subject to acetylation and undergoes alternative splicing, producing multiple isoforms that may fine-tune enzymatic activity.
• Classified as a transferase and a member of the purine salvage enzyme family; its 3D structure has been resolved, facilitating structure-function studies.

Experimental Guidance and Technical Tips

• For Western blot (WB), the immunogen corresponds to the N-terminal region (aa 1-283) of MTAP; an expected band near 31 kDa should be observed in lysates from human, mouse, or rat cells. Consider using a ubiquitous positive control such as HEK293 or HeLa.
• For immunofluorescence (IF/ICC), verify fixation and permeabilization protocols, as MTAP’s dual cytoplasmic/nuclear localization may require optimized conditions.
• In ELISA applications, the polyclonal nature allows use as either capture or detection antibody; however, thorough pairing and titre optimization are recommended.
• Given the widespread expression of MTAP, tissues with high polyamine metabolism (e.g., liver, kidney) may yield stronger signals.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw-material procurement, linking diagnostic manufacturers and research labs with high-quality antibodies and reagents. This anti-MTAP polyclonal antibody supports investigations into polyamine metabolism and cancer metabolism, and we can additionally supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing. Let CamelBio streamline your transition from concept to clinic with reliable, one-stop access.