Anti-MTAP Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q13126

Core Product Specifications and Parameters

Biological Background: MTAP Function and Localization

• MTAP (S-methyl-5'-thioadenosine phosphorylase) is also known as MTA phosphorylase, MTAPase, and is encoded by the MTAP gene (MSAP).
• It catalyzes the phosphorolytic cleavage of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate, playing a critical role in polyamine metabolism and the methionine salvage pathway. Related references: PMID:3091600, PMID:8687427, PMID:10404592
• MTAP exhibits broad substrate specificity, particularly for 6-aminopurine nucleosides.
• It is localized in both the cytoplasm and nucleus.
• MTAP is ubiquitously expressed across human tissues.
• The protein undergoes post-translational modifications including acetylation, and alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide corresponding to amino acids 184-283 of human MTAP. Consider using peptide competition assays to confirm signal specificity.
• For Western blot (WB), the predicted molecular weight is 31 kDa; include proper loading controls and validate in relevant cell or tissue lysates.
• For IHC-P, optimize antigen retrieval methods (e.g., heat-induced epitope retrieval) and validate with appropriate positive and negative tissue controls.
• In IF/ICC, ensure adequate permeabilization to detect nuclear and cytoplasmic pools of MTAP.
• For IP and ELISA, confirm antibody suitability under native and denaturing conditions as needed for your assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive IVD raw material solutions. This anti-MTAP monoclonal antibody is suitable for studies in polyamine metabolism and oncology research. Beyond single antibodies, CamelBio offers validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, streamlining your development from concept to clinic.