Anti-MTA1 Rabbit Polyclonal Antibody for WB and ELISA - Q13330

Core Product Specifications and Parameters

Biological Background: MTA1 Function and Localization

• MTA1 is a transcriptional coregulator that can act as both a transcriptional corepressor and coactivator, modulating gene expression in various physiological and pathological contexts. Related references: PMID:16617102 PMID:17671180 PMID:17922032
• As a core component of the NuRD histone deacetylase complex, MTA1 remodels chromatin and represses transcription of key target genes including BRCA1, ESR1, TFF1, and CDKN1A. Related references: PMID:16428440 PMID:28977666 PMID:17671180 PMID:17922032 PMID:24413532
• Independently of the NuRD complex, MTA1 functions as a coactivator for BCAS3, SUMO2, and WNT1, highlighting its versatility in transcriptional regulation. Related references: PMID:16617102 PMID:17671180 PMID:21965678
• MTA1 regulates p53-dependent and -independent DNA repair following genotoxic stress by inhibiting p53 ubiquitination and degradation, thereby maintaining genomic stability. Related references: PMID:19837670
• MTA1 plays an essential role in the regulation of the circadian clock by modulating CLOCK-BMAL1 transcriptional activity and SIRT1 expression (by similarity).
• Subcellularly, MTA1 is found in the nucleus, cytoplasm, nuclear envelope, and cytoskeleton, and it can sequester ESR1 in the cytoplasm to enhance its non-genomic responses.
• MTA1 is widely expressed with higher levels in brain, liver, kidney, and cardiac muscle, and is markedly upregulated in a wide variety of cancers, where its expression correlates with tumor aggressiveness and metastasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 600–700) of human MTA1, which is conserved in mouse, supporting the observed cross-reactivity.
• For Western blot, a band at approximately 81 kDa is expected; consider enriching nuclear or cytoplasmic fractions to improve detection, as MTA1 localizes to multiple compartments.
• Post-translational modifications such as acetylation and phosphorylation may cause band shifts; validate the antibody in your specific sample system.
• This antibody is suitable for ELISA; optimal working dilutions should be determined empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop supply of IVD raw materials, including validated monoclonal and polyclonal antibodies, antigen raw materials, and ancillary reagents, supporting projects from early research to clinical application. For MTA1-related studies, we offer tailored sourcing of rare-target antibody pairs and bulk reagents to streamline your diagnostic assay development. Our comprehensive portfolio and technical consulting help bridge the gap between breakthrough discovery and reliable commercial use.