Anti-MT-ND6 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P03923

Core Product Specifications and Parameters

Biological Background: MT-ND6 Function and Localization

• MT-ND6 is a core subunit of mitochondrial respiratory chain Complex I (NADH-ubiquinone oxidoreductase), essential for its catalytic activity and assembly; it catalyzes electron transfer from NADH to ubiquinone. Related references: PMID:14595656, PMID:8644732
• The protein localizes to the mitochondrion inner membrane, consistent with its role in oxidative phosphorylation.
• It contains multiple transmembrane helices, functioning as a transmembrane translocase component.
• Genetic variations in MT-ND6 are linked to mitochondrial diseases, including Leber hereditary optic neuropathy, Leigh syndrome, MELAS syndrome, and dystonia.
• Belongs to the complex I ND6 subunit family; its molecular weight is approximately 18.6 kDa (predicted) or 19 kDa (observed).
• The MT-ND6 protein has been identified through proteomics and is part of the reference human proteome.
• Post-translational modifications and disease-associated variants may impact complex I assembly and electron transport efficiency.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight of MT-ND6 is approximately 19 kDa; use mitochondrial-enriched samples or purified recombinant protein as a positive control.
• When performing immunohistochemistry (IHC-P), consider testing different antigen retrieval methods to optimize staining in your specific tissue type.
• This antibody may cross-react with mouse and rat orthologs, as indicated by sequence homology; validate specificity in the relevant species.
• For ELISA, consider using the immunizing peptide or recombinant MT-ND6 protein to establish assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner in IVD raw-material sourcing, providing a comprehensive portfolio of antibodies, antigens, and ancillary reagents from early-stage development to clinical manufacturing. The MT-ND6 polyclonal antibody is part of our mitochondrial research collection, supporting in-depth studies on Complex I function and related pathologies. For customized bulk orders or assistance in sourcing rare target raw materials, our team is ready to help accelerate your project timelines.