Anti-MT-ND6 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P03923

Core Product Specifications and Parameters

Biological Background: MT-ND6 Function and Localization

• MT-ND6, also known as NADH-ubiquinone oxidoreductase chain 6 or NADH dehydrogenase subunit 6, is a core subunit of mitochondrial respiratory chain Complex I, encoded by the mitochondrial MT-ND6 gene.
• It is essential for the catalytic activity and assembly of Complex I, which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Related references: PMID:14595656 PMID:8644732
• MT-ND6 belongs to the mitochondrial NADH dehydrogenase family, part of a multi-protein complex with subunits encoded by both nuclear and mitochondrial genomes.
• The protein localizes to the mitochondrial inner membrane, where it is integrated via multiple transmembrane helices.
• UniProt keywords highlight its involvement in primary mitochondrial diseases, including Leber hereditary optic neuropathy, Leigh syndrome, MELAS syndrome, and dystonia; it is also a reference proteome identified by proteomics.
• Its calculated molecular weight is approximately 18.6 kDa, but it commonly migrates at around 19 kDa in SDS-PAGE.

Experimental Guidance and Technical Tips

• Since MT-ND6 is a mitochondrial inner membrane protein, consider enriching mitochondrial fractions or using mitochondrial-specific lysis buffers for optimal detection.
• The immunogen corresponds to a conserved internal peptide (amino acids 134–150); verify cross-reactivity if working with other species, as the antibody is validated only for human.
• For WB, recommended loading controls should account for mitochondrial protein abundance; validate exposure times and antibody dilutions in your specific sample system.
• In IHC-P, antigen retrieval may be necessary; test on known positive and negative control tissues to establish staining specificity.
• ELISA applications may require optimization of coating conditions and detection protocols using recombinant MT-ND6 or peptide standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for one-stop IVD raw material sourcing, offering high-quality monoclonal antibodies like this MT-ND6 Rabbit mAb for mitochondrial disorder and Complex I research. Beyond individual antibodies, we supply validated antibody pairs, optimized polyclonal reagents, bulk ancillary raw materials, and custom sourcing for rare targets to support your diagnostic development from concept to clinic.