Anti-MT-ND5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P03915

Core Product Specifications and Parameters

Biological Background: MT-ND5 (NADH-ubiquinone oxidoreductase chain 5) Function and Localization

• MT-ND5 encodes the NADH-ubiquinone oxidoreductase chain 5 protein, also known as NADH dehydrogenase subunit 5, a core component of the mitochondrial respiratory chain.
• Functions as a core subunit of mitochondrial Complex I, catalyzing electron transfer from NADH to ubiquinone, and is essential for complex I catalytic activity and assembly. Related references: PMID:15250827
• Located in the mitochondrial inner membrane, featuring multiple transmembrane helices that anchor the complex.
• Classified under respiratory chain, translocase, and transport keywords, reflecting its role in proton translocation coupled to electron transport.
• Associated with several primary mitochondrial diseases, including Leber hereditary optic neuropathy, Leigh syndrome, and MELAS syndrome, when mutated.
• The protein has a molecular weight of approximately 67 kDa (based on UniProt annotation) and is identified through proteomics approaches.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the 500-600 amino acid region. Consider verifying antibody specificity through peptide blocking assays or knockdown/knockout models.
• For Western blotting, a band at ~67 kDa is expected. Mitochondrial-enriched lysates may improve detection. Validate in the relevant sample system.
• In IHC-P, optimize antigen retrieval conditions and test on tissues with known high mitochondrial content (e.g., heart, skeletal muscle).
• For ELISA, use as a capture or detection reagent; cross-reactivity with mouse and rat allows multispecies studies, but epitope conservation should be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material sourcing for diagnostic manufacturers and research laboratories. This MT-ND5 antibody is part of our mitochondrial research portfolio, supporting assay development for metabolic and mitochondrial disorders. Beyond antibodies, we supply validated antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to accelerate your project from concept to clinic.