Anti-MT-ND5 Rabbit Polyclonal Antibody for WB and ELISA - P03915

Core Product Specifications and Parameters

Biological Background: MT-ND5 Function and Localization

• MT-ND5 (NADH dehydrogenase subunit 5) is a core subunit of mitochondrial Complex I (NADH-ubiquinone oxidoreductase), which initiates the electron transport chain.
• It catalyzes the transfer of electrons from NADH to ubiquinone, a critical step in oxidative phosphorylation (PubMed:15250827). Related references: PMID:15250827
• Essential for both the catalytic activity and assembly of Complex I (PubMed:15250827). Related references: PMID:15250827
• Located in the mitochondrial inner membrane, where it functions as a multi-pass transmembrane protein.
• Encoded by mitochondrial DNA (MT-ND5 gene), mutations in this gene are linked to mitochondrial disorders including Leber hereditary optic neuropathy (LHON), Leigh syndrome, and MELAS syndrome.
• The human protein has a predicted molecular weight of approximately 67 kDa.

Experimental Guidance and Technical Tips

• For Western blot, consider enriching mitochondrial fractions from human, mouse, or rat tissues to improve detection of the ~67 kDa band.
• Validate antibody specificity using a blocking peptide (corresponding to the immunogen sequence) or by knockdown/overexpression models.
• In ELISA applications, pre-test with recombinant MT-ND5 or peptide to confirm binding and optimize coating concentrations.
• Because MT-ND5 is highly conserved, cross-reactivity with other species may exist; always verify with your target sample.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized provider of IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to validated product. For mitochondrial targets such as MT-ND5, we offer validated antibody pairs, high-performance monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare targets. Our integrated supply chain helps streamline your mitochondrial disease assay development and biomarker validation workflows.