Anti-MT-ND4L Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P03901

Core Product Specifications and Parameters

Biological Background: MT-ND4L Function and Localization

• MT-ND4L encodes the mitochondrial NADH-ubiquinone oxidoreductase chain 4L, a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). Related references: PMID:28844695
• It catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor, contributing to the proton motive force across the inner mitochondrial membrane. Related references: PMID:28844695
• The enzyme's membrane arm, where ND4L resides, is embedded in the lipid bilayer and is involved in proton translocation. Related references: PMID:28844695
• MT-ND4L is localized to the mitochondrion inner membrane.
• The protein is associated with Leber hereditary optic neuropathy, a disease linked to mitochondrial complex I dysfunction.
• It spans the membrane with multiple transmembrane helices, functioning as a translocase in the respiratory chain.

Experimental Guidance and Technical Tips

• The immunogen is designed against the C-terminal region (amino acids 40 to end) of human MT-ND4L. This region may be accessible in denaturing conditions for WB. Consider optimizing antigen retrieval and blocking conditions for IF/ICC.
• The antibody demonstrates cross-reactivity with mouse and rat, making it suitable for studies in these species. Validate performance in the relevant sample system.
• For ELISA, consider using the synthetic peptide immunogen as a positive control to assess antibody binding.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material sourcing partner, CamelBio supports diagnostic manufacturers and research labs with high-quality antibodies and reagents. This anti-MT-ND4L polyclonal antibody enables the study of mitochondrial Complex I function and related disorders, fitting into our catalog of validated tools for mitochondrial research. Explore our extensive portfolio of antibody pairs, optimized polyclonal and monoclonal antibodies, and flexible bulk supply options to accelerate your assay development from concept to clinic.