Anti-MT-ND4 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P03905

Core Product Specifications and Parameters

Biological Background: MT-ND4 (NADH Dehydrogenase Subunit 4) Function and Localization

• MT-ND4 encodes NADH-ubiquinone oxidoreductase chain 4, also known as NADH dehydrogenase subunit 4, a core subunit of mitochondrial respiratory chain complex I.
• The protein is essential for the catalytic activity and assembly of complex I, which catalyzes electron transfer from NADH to ubiquinone, driving proton translocation across the inner membrane. Related references: PMID:15250827, PMID:8344246, PMID:8644732
• MT-ND4 is encoded by the mitochondrial genome, and mutations are associated with Leber hereditary optic neuropathy (LHON), MELAS syndrome, and dystonia.
• It localizes to the mitochondrial inner membrane, consistent with its role in oxidative phosphorylation.
• Keywords assigned to this protein include: 3D-structure, Disease variant, Electron transport, NAD, Primary mitochondrial disease, Translocase, Transmembrane helix, and Ubiquinone.
• The predicted molecular weight of full-length MT-ND4 is approximately 51.6 kDa; however, the mature form may migrate around 52 kDa on SDS-PAGE, consistent with product specifications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the C-terminal region (amino acids 401–459) of human MT-ND4, which may confer high specificity across applications.
• For Western blot (WB), consider using mitochondrial-enriched lysates or tissue extracts to improve detection, given the protein’s mitochondrial localization.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval steps may be required, and co-staining with a mitochondrial marker is recommended to confirm localization.
• In ELISA, the antibody can be used as a capture or detection reagent; optimal pairing with secondary antibodies or other sandwich components should be validated experimentally.
• Cross-reactivity with mouse and rat has been reported, but users should verify performance in these species based on sequence homology and sample preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing solution for IVD raw materials and technical services, supporting diagnostic manufacturers from concept to clinic. For mitochondrial complex I research targeting MT-ND4, we supply validated rabbit polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to drive robust assay development. Our global supply network also enables access to rare-target raw materials, helping you accelerate projects in energy metabolism, neurodegeneration, and inherited mitochondrial disorders.