Anti-MT-ND3 Rabbit Polyclonal Antibody for WB - P03897

Core Product Specifications and Parameters

Biological Background: MT-ND3 Function and Localization

• MT-ND3 encodes the NADH-ubiquinone oxidoreductase chain 3, a core subunit of mitochondrial respiratory chain Complex I.
• It catalyzes electron transfer from NADH through the respiratory chain using ubiquinone as an electron acceptor, and is essential for the catalytic activity of Complex I. Related references: PMID:25118196
• Localizes to the mitochondrial inner membrane.
• It is a transmembrane protein with multiple transmembrane helices.
• Mutations in MT-ND3 are associated with mitochondrial diseases such as Leigh syndrome.
• Part of the oxidative phosphorylation system, playing a key role in electron transport and proton translocation.
• Identified as a reference proteome component and detected via proteomics identification.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1–70) of human MT-ND3, which may confer high specificity; confirm reactivity in your target species if using mouse or rat samples.
• For WB, consider using mitochondrial-enriched lysates to enhance detection of this 13 kDa protein.
• For IHC-P and IF/ICC, validate the antibody in the relevant tissue/cell type, as MT-ND3 is ubiquitously expressed but enriched in metabolically active tissues.
• For IP, ensure mild detergent lysis to preserve complex integrity if studying Complex I interactions; validate co-immunoprecipitated proteins.
• ELISA performance should be verified with appropriate peptide or recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a streamlined one-stop platform for IVD raw material procurement, including validated polyclonal antibodies like this anti-MT-ND3 antibody for mitochondrial disease research. Beyond antibodies, we supply bulk ancillary reagents and assist in sourcing rare target raw materials, covering every stage from concept to clinic. Let CamelBio bridge your sourcing gaps and accelerate your diagnostic development.