Anti-MT-CYB Rabbit Polyclonal Antibody for WB and ELISA - P00158

Core Product Specifications and Parameters

Biological Background: MT-CYB Function and Localization

• MT-CYB encodes Cytochrome b, also known as Complex III subunit 3, which is a core component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) in the mitochondrial respiratory chain.
• The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c, contributing to the generation of a proton gradient across the mitochondrial membrane that drives ATP synthesis.
• Cytochrome b is an integral transmembrane protein located in the mitochondrial inner membrane.
• It contains heme groups and iron-sulfur clusters, binding heme and metal ions essential for its electron transport function.
• The protein spans the membrane with multiple transmembrane helices, as indicated by the keyword "Transmembrane helix".
• Keywords associated with MT-CYB include 3D-structure, electron transport, heme, iron, membrane, metal-binding, mitochondrion, mitochondrion inner membrane, respiratory chain, transport, ubiquinone.
• Note: The antibody has cross-reactivity with human and rat MT-CYB, indicating conserved epitopes across these species.

Experimental Guidance and Technical Tips

• For Western blotting, a band around 43 kDa is expected; consider using mitochondrial-enriched lysates for optimal detection.
• Validate specificity in the relevant sample systems (human, mouse, rat) and include appropriate controls.
• For ELISA, the immunizing synthetic peptide can serve as a positive control to assess antibody binding.
• Optimization of antibody dilution may be necessary depending on sample type and assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. This anti-MT-CYB antibody is part of our mitochondrial research reagents, supporting diagnostic developers and researchers in respiratory chain studies. Our one-stop sourcing services also cover rare-target raw material procurement and technical consulting, helping you accelerate from concept to clinic.