Anti-MT-CYB Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P00156

Core Product Specifications and Parameters

Biological Background: MT-CYB Function and Localization

• MT-CYB encodes Cytochrome b, also known as Complex III subunit 3, a core subunit of the ubiquinol-cytochrome c reductase (complex III) in the mitochondrial electron transport chain.
• As part of complex III, MT-CYB mediates electron transfer from ubiquinol to cytochrome c, coupling this to proton translocation across the inner mitochondrial membrane, generating the proton gradient essential for ATP synthesis.
• Cytochrome b contains two heme groups (b562 and b566) that participate in electron transfer, with conserved histidine residues coordinating the heme iron atoms.
• Mutations in MT-CYB are associated with human mitochondrial disorders, including Leber hereditary optic neuropathy, cardiomyopathy, cataracts, and deafness, reflecting its critical role in oxidative phosphorylation.
• The protein is encoded by the mitochondrial genome and localized to the mitochondrial inner membrane with eight transmembrane helices, typical of the cytochrome b family.
• Additional keywords: MT-CYB is a membrane-bound metalloprotein with roles in ubiquinone binding and respiratory chain function.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to amino acids 100-200 of human MT-CYB (YP_003024038.1). Consider sequence alignment with mouse and rat orthologs to predict cross-reactivity.
• For Western blot (WB), a band of approximately 43 kDa is expected. Validate antibody specificity in your sample system using positive and negative controls.
• For IHC-P and IF/ICC, antigen retrieval may be required. Test antibody dilutions in your specific tissue or cell type, as mitochondrial compartments may need optimized permeabilization.
• ELISA applications may involve direct or capture formats; pairing with a suitable detection system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing of high-quality IVD raw materials, including polyclonal antibodies like this anti-MT-CYB antibody, for diagnostic manufacturers and researchers. Our portfolio encompasses mitochondrial biology and respiratory chain targets, supported by bulk supply and custom sourcing services. With a commitment to streamlining your pipeline, we offer technical consultation and reliable material supply to accelerate your projects.