Anti-MT-ATP8 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P03928

Core Product Specifications and Parameters

Biological Background: MT-ATP8 Function and Localization

• MT-ATP8 encodes ATP synthase F(0) complex subunit 8, also known as A6L or F-ATPase subunit 8, a core component of the mitochondrial oxidative phosphorylation system.
• It is an integral subunit of the membrane F(0) domain of ATP synthase (Complex V), which produces ATP from ADP using the proton gradient generated by the electron transport chain. Related references: PMID:37244256
• As part of the F(0) proton channel, MT-ATP8 participates in the rotary mechanism that couples proton translocation across the inner mitochondrial membrane to ATP synthesis in the F(1) catalytic domain.
• The protein is localized to the mitochondrial membrane (UniProt: P03928).
• It is predicted to contain a single transmembrane helix, anchoring it within the lipid bilayer (UniProt keywords: Transmembrane, Transmembrane helix).
• Post-translational acetylation has been identified, potentially regulating its function (UniProt keyword: Acetylation).
• Disease-associated variants in MT-ATP8 are linked to primary mitochondrial disorders, including cardiomyopathy (UniProt keywords: Cardiomyopathy, Disease variant, Primary mitochondrial disease).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide derived from the N-terminal region (amino acids 1–68) of human MT-ATP8. This peptide-based design may confer reactivity against both denatured and native protein targets; however, validation in the relevant experimental system is advised.
• Given its small molecular weight (8 kDa), ensure optimal transfer and detection conditions during Western blotting, such as using high-retention membranes and extended transfer times for low-molecular-weight proteins.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and permeabilization steps to access the mitochondrial membrane-localized epitope.
• The antibody is reported to cross-react with mouse and rat orthologs based on sequence similarities. Independent verification is recommended if species-specific detection is critical.
• ELISA performance may be influenced by coating antigen and buffer conditions; titration experiments are suggested to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic developers and life science researchers a streamlined pathway to procure high-quality raw materials, including this MT-ATP8 polyclonal antibody, for mitochondrial and ATP synthase-focused assays. Our portfolio extends to validated antibody pairs, recombinant antigens, and ancillary reagents tailored for IVD applications, supplemented by expert sourcing support for rare and custom targets. Partner with CamelBio to accelerate your project from early feasibility to commercial readiness.