Anti-MT-ATP6 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P00846

Core Product Specifications and Parameters

Biological Background: MT-ATP6 Function and Localization

• MT-ATP6 encodes the ATP synthase F(0) complex subunit a, also known as F-ATPase protein 6, a core component of complex V of the mitochondrial respiratory chain.
• Subunit a collaborates with subunit c (ATP5MC1) to form the proton-conducting channel in the F(0) domain, driving ATP synthesis via the rotary catalytic mechanism.
• The protein resides in the mitochondrial inner membrane and spans it multiple times, creating two half-channels for proton translocation via a Grotthuss mechanism.
  Related references: PMID:37244256
• Mutations in MT-ATP6 are linked to mitochondrial disorders including Leber hereditary optic neuropathy, Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa (as noted in UniProt disease keywords).
• The protein is highly conserved across human, mouse, and rat, suggesting cross-reactivity potential.
• With a molecular weight of approximately 25 kDa (24817 Da by calculation), it migrates as a discrete band in SDS–PAGE under appropriate conditions.

Experimental Guidance and Technical Tips

• The immunogen targets the N‑terminal 1‑100 amino acid sequence of human MT‑ATP6, which is predicted to face the mitochondrial matrix. Therefore, membrane permeabilization is essential for epitope access in IF/ICC and IHC‑P.
• For Western blotting, a band near 25 kDa is expected. Enrichment of mitochondrial fractions can improve signal‑to‑noise ratio.
• In immunostaining, co‑localization with a mitochondrial marker (e.g., MitoTracker, anti‑ATP5A) is advisable to confirm specificity.
• Antigen retrieval methods (heat‑induced or enzymatic) may be required for formalin‑fixed paraffin‑embedded sections in IHC‑P; test on tissues with abundant mitochondria (heart, skeletal muscle).
• These recommendations are based on immunogen properties and target biology; each laboratory should establish its own optimized protocol.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier B2B supplier of IVD raw materials, offering this anti‑MT‑ATP6 polyclonal antibody as part of a comprehensive portfolio for mitochondrial disease research and diagnostics. Beyond single antibodies, we provide matched antibody pairs, bulk monoclonals/polyclonals, assay ancillary reagents, and custom sourcing for rare targets. Our expert team supports manufacturers from initial concept through clinical validation, streamlining your path to market.