Anti-MSY2 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y2T7

Core Product Specifications and Parameters

Biological Background: MSY2 Function and Localization

• MSY2 (Y-box-binding protein 2), also known as DBPC, CSDA3, or CONTRIN, is a germ cell-specific member of the Y-box binding protein family that contains a cold-shock domain and is involved in nucleic acid binding.
• It functions as a major constituent of messenger ribonucleoprotein particles (mRNPs) and plays a critical role in regulating the stability and/or translation of germ cell mRNAs.
• MSY2 binds to the Y-box consensus promoter element and also interacts with full-length mRNA in a sequence-independent manner with high affinity, while recognizing short RNA sequences like 5'-UCCAUCA-3' with low affinity and limited specificity.
• The protein shuttles between the cytoplasm and nucleus, and its binding to maternal mRNAs is essential for cytoplasmic retention, thereby linking transcription to mRNA storage and translational delay.
• Tissue expression is prominent in oocytes and testicular germ cells from spermatogonia to spermatocyte stages; it is also detected in placental trophoblasts, vascular smooth muscle, myocardium, and skeletal muscle, but not in most epithelial cells.
• MSY2 is up-regulated in various carcinomas and germ cell tumors, highlighting its potential as a biomarker; post-translational modifications include phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (aa 1-100), suggesting the antibody may recognize the full-length endogenous protein; for WB, a band around 39 kDa is expected based on the predicted molecular weight.
• Given cross-reactivity with mouse, this antibody may be suitable for mouse tissue or cell lysates; however, sequence alignment and experimental validation are recommended to confirm specificity.
• For ELISA, direct coating with the immunogen peptide or recombinant MSY2 protein can be considered; pre-adsorption and blocking steps should be optimized for background reduction.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic developers and researchers with a comprehensive portfolio of IVD raw materials, including this polyclonal antibody targeting the germ-cell regulator MSY2. We supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and offer support in sourcing rare-target raw materials to accelerate your projects in reproductive biology and oncology. Our integrated services help streamline procurement from concept to clinic.