Anti-MST1/STK4 Polyclonal Antibody for WB, ELISA - Q13043

Core Product Specifications and Parameters

Biological Background: MST1/STK4 Function and Localization

• Serine/threonine-protein kinase 4, also known as MST1, KRS2, YSK3, belongs to the STE20-like kinase family.
• Core component of the Hippo signaling pathway, regulating organ size and tumor suppression by phosphorylating LATS1/2, leading to YAP1/WWTR1 inactivation.
• Functions as a stress-activated, pro-apoptotic kinase; caspase-cleavage induces nuclear translocation, chromatin condensation, and DNA fragmentation.
• Phosphorylates histone H2B at Ser14 during apoptosis, and phosphorylates FOXO3 upon oxidative stress, promoting nuclear localization and cell death.
• Inhibits SIRT1-mediated p53 deacetylation, enhancing p53-dependent apoptosis; also acts as an inhibitor of PKB/AKT1.
• Localizes to both cytoplasm and nucleus.
• Ubiquitously expressed, with increased levels observed in prostate cancer.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment spanning the C-terminal region (aa 303–487). The polyclonal antibody may detect full-length MST1/STK4 and caspase-cleaved fragments; consider validating in appropriate apoptotic models.
• For WB, the predicted molecular weight is ~56 kDa. A band near this size should be observed; additional bands may arise from cleavage or post-translational modifications.
• The antibody is confirmed for ELISA. When developing sandwich ELISA assays, consider pairing with a capture antibody that recognizes a different epitope to ensure specificity.
• Cross-reactivity is reported for human, mouse, and rat; always verify performance in the relevant sample matrix and include proper controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop sourcing of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and ancillary reagents. This anti-MST1/STK4 antibody supports research into apoptosis and Hippo signaling pathways, relevant for oncology and cell biology diagnostics. For assay development or bulk requirements, our team can source additional rare targets and technical support.