Anti-MSRB3 Rabbit Monoclonal Antibody for WB and ELISA - Q8IXL7

Core Product Specifications and Parameters

Biological Background: MSRB3 Function and Localization

• MSRB3 (Methionine-R-sulfoxide reductase B3) is also known as DFNB74, reflecting its association with hereditary deafness.
• It catalyzes the reduction of methionine sulfoxide back to methionine in both free and protein-bound forms, protecting cells from oxidative damage.
• Multiple isoforms are produced via alternative splicing; isoform 2 is specifically essential for auditory function.
• MSRB3 localizes to the endoplasmic reticulum and mitochondria, indicating a role in organelle-specific protein repair.
• It is widely expressed across human tissues, consistent with its fundamental function in redox homeostasis.
• Post-translational modifications include acetylation and phosphorylation, and it requires zinc as a cofactor.
• Mutations in MSRB3 cause non-syndromic deafness (DFNB74), underscoring its critical role in hearing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a conserved region (aa 50–192) shared across species, supporting the observed cross-reactivity with human, mouse, and rat.
• For Western blot, a band near 21 kDa is expected, but additional isoforms or post-translational modifications may produce variants in some samples.
• Validate the antibody in your specific sample system, particularly in hearing-related tissues or cell lines, to confirm performance.
• For ELISA applications, consider optimizing coating and detection conditions using standard protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to high-quality IVD raw materials. This anti-MSRB3 monoclonal antibody supports investigations into oxidative stress, methionine metabolism, and hearing loss. Beyond single antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare target sourcing to streamline your assay development pipeline.