Anti-MSRB1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9NZV6

Core Product Specifications and Parameters

Biological Background: MSRB1 Function and Localization

• MSRB1, also known as Selenoprotein X (SelX), is a methionine-R-sulfoxide reductase (MsrB) that specifically reduces methionine (R)-sulfoxide back to methionine, playing a critical role in antioxidant defense and redox regulation.
• It acts as a regulator of actin assembly by reducing methionine (R)-sulfoxide groups on actin introduced by MICAL monooxygenases (MICAL1, MICAL2, MICAL3), thereby promoting actin filament repolymerization and cytoskeleton dynamics.
• MSRB1 plays a role in innate immunity by reducing oxidized actin in macrophages, facilitating actin repolymerization and contributing to immune cell function.
• The protein contains a selenocysteine (Sec) residue at its active site, which is essential for its enzymatic activity; it also binds zinc, indicative of its metal-binding properties.
• Subcellularly, MSRB1 localizes to the cytoplasm, nucleus, and cytoskeleton, consistent with its roles in actin regulation and nuclear redox balance.
• Keywords associated with MSRB1 include oxidoreductase, immunity, innate immunity, metal-binding, zinc, selenocysteine, and reference proteome, highlighting its multifaceted biological significance.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-94 of human MSRB1. The antibody is raised in rabbit and is polyclonal; batch-to-batch consistency may vary, so consider validating each new lot in your assay system.
• For Western blot (WB) under reducing conditions, a band around 13 kDa is expected corresponding to the predicted molecular weight of MSRB1; however, perform appropriate controls and consider potential post-translational modifications or isoforms.
• This antibody has been tested in WB, IHC-P, IF/ICC, and ELISA applications, and shows cross-reactivity with human, mouse, and rat samples; orthogonal validation is recommended for each application and species.
• In IHC-P and IF/ICC, optimization of antigen retrieval, blocking, and antibody dilution may be necessary; use relevant positive and negative controls to ensure specificity.
• For ELISA, pair with a suitable capture or detection antibody as needed, and calibrate using recombinant MSRB1 protein for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes comprehensive access to premium IVD raw materials, including this MSRB1 polyclonal antibody. Our one-stop sourcing platform streamlines procurement of validated antibody pairs, bulk ancillary reagents, and rare target raw materials. For research focused on oxidative stress, actin cytoskeleton regulation, or innate immunity, CamelBio is your trusted partner from concept to clinic.