Anti-MSRA Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UJ68

Core Product Specifications and Parameters

Biological Background: MSRA Function and Localization

• MSRA (Mitochondrial peptide methionine sulfoxide reductase), also known as PMSR, is a repair enzyme that catalyzes the reversible reduction of methionine sulfoxide residues in proteins back to methionine, restoring protein function damaged by oxidative stress. Related references: PMID:10452521, PMID:12039877
• It plays a crucial role in maintaining lens transparency by repairing oxidized methionine in alpha-crystallin (CRYAA/CRYAB) and cytochrome c (CYCS) (By similarity).
• The enzyme is widely expressed, with highest levels in adult kidney and cerebellum, and moderate in liver, heart ventricles, bone marrow, and hippocampus.
• MSRA localizes to multiple subcellular compartments including the mitochondrial matrix, cytoplasm, nucleus, and membrane, consistent with its role in repairing proteins across organelles.
• The protein undergoes alternative splicing, alternative promoter usage, and alternative translation initiation, generating multiple isoforms with distinct N-termini and targeting signals.
• MSRA contains a redox-active cysteine center and forms a transient disulfide bond during catalysis. It is acetylated and myristoylated, modifications that may regulate its activity or localization.
• Keywords from UniProt: Acetylation, Alternative initiation, Alternative promoter usage, Alternative splicing, Cytoplasm, Disulfide bond, Lipoprotein, Membrane, Mitochondrion, Myristate, Nucleus, Oxidoreductase, Redox-active center, Transit peptide.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein spanning amino acids 24–235 of human MSRA (NP_036463.1), covering the mature catalytic domain of the protein. Consider this region for antibody specificity validation.
• For Western blot (WB), the predicted molecular weight is 26 kDa. Because MSRA has alternative isoforms and localizes to multiple compartments, validate band specificity using relevant knockout or knockdown controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), prefixation/permeabilization conditions should be optimized for the target’s presence in membrane, mitochondrial, and nuclear fractions. Use appropriate organelle markers to confirm subcellular distribution.
• ELISA may be used for quantitative detection; ensure the coating antigen matches the immunogen sequence for optimal sensitivity.
• Cross-reactivity is validated for human, mouse, and rat; for other species, check sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a comprehensive sourcing solution for IVD raw materials, technical services, and consulting—from concept to clinic. This MSRA antibody supports research in oxidative stress, protein repair, and age-related diseases. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare-target raw materials to accelerate your assay development.